GPC软件现场培训教材

󰀉󰀆󰀀󰀀Agilent󰀀GPC󰀀Software󰀀󰀀󰀀󰀀󰀀ந󰀃󰀄ၕ󰀆󰀇󰀁󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀sÂ󰀋󰀍ܾ󰀑󰀓ˤ󰀄󰀍q󰀀󰀀󰀀󰀀Page󰀀1󰀀of󰀀16󰀀󰀉󰀆󰀀󰀌󰀐󰀁󰀊󰀀Agilent󰀀1100/1200󰀀LC󰀀󰆔󰀀G1310A()G1312A()G1311A()󰀀󰆔󰀀G1322A/G1379A,󰀀󰀀󰆔󰀀G1313A,󰀀󰀀󰆔󰀀G1316A,󰀀󰀀/󰀀G1362A󰆔󰀀G1314A/BG1315A/B/CG1365A/B/C󰀀󰆔󰀀GPC-SEC󰀀5064-82515064-8252󰀀󰀀󰆔󰀀G2182BA󰀀GPC󰀀Add-onG2182BAG2182AA󰀀󰀀󰀀(G1362A)100%A100%󰀀B;(P/N󰀀0100-1847)()RID󰀀GPC-SEC󰀀(:5064-8251;:5064-8252)demoDemoGPC󰀀󰀀.󰀀Page󰀀2󰀀of󰀀16󰀀󰀉󰀆󰀀.󰀀nj󰀏󰂾󰀀󰀀󰂾󰀀Narrow󰀀Standards󰀀Calibration󰀍󰀀Data󰀀Analysis󰀀󰀀DATA\\SUPPORT1VWD󰀀󰂾󰀀󰀀󰂾󰀀󰀀001-0101.DVWDRI󰀀GPCGPC󰀀SettingGPC󰀀Settings...󰀀GPC󰀀Activate󰀀GPCGPC󰀀󰀀󰀀123Calibration󰀀TableCorrection󰀀Internal󰀀Standard󰀀CorrectionUse󰀀Internal󰀀Standard󰀀󰀀Default.cal󰀀󰀀Page󰀀3󰀀of󰀀16󰀀󰀉󰀆 45M[D]6Mass󰀀Fraction󰀀at󰀀Molar󰀀Mass󰀀at󰀀I(M)[%]󰀀󰀀Delay󰀀󰂾󰀀󰀀GPC󰀀Calculate󰀀GPC󰀀ResultsGPC-Addon󰀀󰀀default.cal󰀀󰀀󰂾󰀀󰀀1.󰀀󰀀󰀀File>NewCalibrationWindow>CalibrationGPC-Addon󰀀GPCElugramRaw Data󰀀󰀀elugram2.󰀀maximumAdd󰀀to󰀀calibration󰀀󰀀x-󰀀find󰀀󰀀Table󰀀󰀀Sample󰀀󰀀Data󰀀file󰀀󰀀Peak󰀀1󰀀󰀀Peak󰀀2󰀀󰀀Peak󰀀3󰀀󰀀Peak󰀀4󰀀󰀀Molecular󰀀mass󰀀󰀀information󰀀󰀀󰀀󰀀PS󰀀ReadyCal󰀀grn󰀀PS󰀀ReadyCal󰀀red󰀀󰀀PS󰀀ReadyCal󰀀wht󰀀󰀀󰀀001-0101󰀀002-0201󰀀003-0301󰀀󰀀󰀀󰀀2󰀀180󰀀000󰀀1󰀀000󰀀000󰀀659󰀀000󰀀󰀀󰀀󰀀246󰀀000󰀀128󰀀000󰀀67󰀀500󰀀󰀀󰀀󰀀32󰀀500󰀀18󰀀100󰀀9󰀀130󰀀󰀀󰀀󰀀3󰀀420󰀀1󰀀620󰀀374󰀀Page 4 of 16 󰀉󰀆 324󰀀4.󰀀󰀀(002-0201.D)GPC󰀀Calculate󰀀GPC󰀀Results󰀀5.󰀀GPC󰀀AddonElugramXFind󰀀Maximum1Add󰀀to󰀀calibration󰀀6󰀀2󰀀󰀀4󰀀7.󰀀󰀀003-0301.D,󰀀4󰀀6󰀀12Calibration8.󰀀PSS󰀀Poly󰀀5**File>Save󰀀Asps_thfCHEM32\\GPC\\CALIB󰀀**󰀀󰀀󰀀󰀀Page 5 of 16 󰀉󰀆 󰀀󰀏󰀌 󰂾󰀀󰀀󰂾󰀀 Data󰀀Analysis󰀀󰀀DATA\\SUPPORT1󰀀󰂾󰀀󰀀SIG10004.DVWD󰀀GPC>󰀀GPC-Settings...ps_thf.calps_thf.calConfigure󰀀Print󰀀Interactive󰀀Screen󰀀ReviewMultipeak󰀀EvaluationGPCDefault.cal.󰀀…Browse,CHEM32\\GPC\\CALIBReport󰀀Settings󰀀Print󰀀results󰀀󰀀󰀀4.󰀀󰀀GPC󰀀Calculate󰀀GPC󰀀ResultsGPC-Addon󰀀ps-thf.cal󰀀Page 6 of 16 󰀉󰀆 GPC󰀀SettingsElugramRaw󰀀DataMass󰀀Distribution󰀀󰀀󰀀󰀀󰀀Page 7 of 16 󰀉󰀆 󰀎󰀌ඩ SUPPORT1PMMA004-0401.D󰀀006-0601.DPMMA Table󰀀 005-0501.DRID254nm 󰀀 Sample󰀀 Data󰀀file Peak󰀀1 Peak󰀀2 Peak󰀀3 Peak󰀀4 Molar mass information 󰀀󰀀PMMA󰀀RdyCal󰀀grn󰀀PMMA󰀀RdyCal󰀀red 004-0401 005-0501 2 740 000 903 000 306 000 103 000 31 800 10 900 1 960 1 020 PMMA󰀀RdyCal󰀀wht 006-0601 579 000 58 700 5 090 505 󰀀󰀀󰀀SIG1006.DMn󰀀=󰀀45󰀀000󰀀DMw󰀀=󰀀110󰀀000󰀀DMnMwMz󰀀=󰀀240󰀀000󰀀D󰀀Page 8 of 16 󰀉󰀆 . ǔ󰀃󰀅󰀋Guided Broad Calibration󰀉 GPC󰂾󰀀GPC󰀀 󰂾󰀀 󰂾󰀀File󰀀 Mw󰀀Mn󰀀WindowCalibration󰀀Calibration󰀀 OpenSupportps_thf_RID.cal Page 9 of 16 󰀉󰀆 󰂾󰀀󰀀󰀀󰀀󰀀 󰂾󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀Calibration󰀀OK 󰀀Guided󰀀Broad󰀀Calibration󰀀Guided󰀀broad󰀀calibration:󰀀Step󰀀1󰀀Open󰀀stored󰀀overlay elugram󰀀dataUse󰀀Actual󰀀 Use󰀀actual󰀀elugram󰀀dataChemStationGPC󰀀Addon󰀀Data/Support1SIG10006.DOverlay󰀀1󰀀ChemStationData󰀀AnalysisGPC>Calculate󰀀GPC󰀀Results󰀀Window>Raw󰀀Data2󰀀GPCOverlayPMMASIG10006.DGPC󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀Page 10 of 16 󰀉󰀆 󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀3󰀀Window>ElugramInformationOverlayOverlayOverlayOverlay1Overlay󰀀>󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀Mw5overlayOverlay>Delete󰀀all󰀀curvesOverlay>OverlaySave󰀀asbroad.addMn󰀀Page 11 of 16 󰀉󰀆 󰂾󰀀 󰂾󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀󰀀 󰂾󰀀Open󰀀stored󰀀overlayBroad_Demo_PMMA.ADD MnMwPMMAData\\Support1overlayoverlayGuided󰀀Broad󰀀Calibration:Step󰀀2Mw110󰀀000Weight1MnMw OKMn󰀀 45󰀀000󰀁 󰀀Guided󰀀Broad󰀀Calibration󰀀Step3 •󰀀ABABGPC󰀀,󰀀p/n󰀀G2182-90020Basic󰀀Theory󰀀of󰀀GPCB󰀀󰀀󰀀•󰀀Grid󰀀Eval.AB󰀀A󰀀Start󰀀Calculation Mn-,󰀀Mw-Broad_Demo_PMMA.ADD.add 8Overlay.add 󰀀ps_thf_rid.cal.add Calc. 1Page 12 of 16 󰀉󰀆 MnMw 󰂾 ABMnMwGrid Eval. Mark-HouwinkGPC G2182-90020 Basic Theory of GPC, subchapter Calibration 󰂾 Guided Broad Calibration Step 4 Mark-HouwinkGivenMark-Houwink CalculatedMark-HouwinkMark-Houwink OKAB Page 13 of 16 󰀉󰀆 󰂾 OK calibration table󰂾 Yes demo_gpc.calOverlayCreate new Broad.calFile>Save as Page 14 of 16 󰀉󰀆 ˥󰀅󰀆󰀄Universal Calibration󰀃 Mark-HouwinkMark-Houwink 󰂾 CalibrationCalibration>SettingsK=0.01363ml/ga =0.714ps_thf.calTHFMark-Houwink Mark-Houwink 󰂾 󰂾 Calibration Universal CalibrationUniversal calibrationCalculate tranformationPMMATHFMark-Houwink K = 0.01298 ml/ga = 0.688PMMA Page 15 of 16 󰀉󰀆 󰂾 󰂾 **OKFilePMMASave AsYesUNIVERSAL.CAL Mark-Houwink KaPage 16 of 16